Composite

Part:BBa_K2070004:Design

Designed by: iGEM UT-Tokyo 2016   Group: iGEM16_UT-Tokyo   (2016-10-09)


ecf35 inducible GFP reporter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 751


Design Notes

BBa_K2070008 (sigma30 promoter) was ligated with BBa_E0240 so that its activity could be reported in terms of RPU.


Source

Assembly

References

Rhodius, V. A., Segall-Shapiro, T. H., Sharon, B. D., Ghodasara, A., Orlova, E., Tabakh, H., Voigt, C. A. (2014, 04). Design of orthogonal genetic switches based on a crosstalk map of s, anti- s, and promoters. Molecular Systems Biology, 9(1), 702-702. doi:10.1038/msb.2013.58